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A freeze-dried aqueous extract(BRM: biological response modifier) from Enterococcus faecalis 2001, lacking fungal products and designated Enterococcus faecalis 2001, was used in this study. Because a lymphocyte/polymorph(L/P)-enhancing activity was fonud when mice were treated with 200 g of BRM(p＜0.05), the extract was examined for its anti-neoplastic effects against Meth A fibrosarcoma in mice, and for analgesic action based on in various animal models. The mice were inoculated with 1 106cells and 2 105cells directly in the right and left flank, respectively, and the anti-neoplastic effects of intratumoral administration of BRM against syngeneic Meth A fibrosarcomas in BALB/c mice were examined. BRM at 1mg/0.1ml in isotonic sodium chloride soultion, was administered into the right neoplasm at 3, 4, and 5 days after tumor implantation. The dimensions of the tumor were measured from day 3 until day 25 after tumor implantation, and a significant reduction in tumor size was observed from day 21 until day 25. The pain-relieving effects of BRM were tested using the acetic acid induced writhing method. At 1,000mg/kg, BRM exerted a significant analgesic effect when compared to the control group.
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